Endometrial dating based on histomorphology blake lively dating ryan

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All equipment and food entering the barrier was autoclaved. All experiments were carried out in accordance with German laws for animal protection and with permission of the state.

Functional impact on angiogenesis was evaluated by density of microvessels and of vessels stabilized by pericytes within the ectopic lesions.The stage of the menstrual cycle was confirmed by histological staging according to Noyes . None of the patients had been diagnosed for endometriosis or had undergone any hormonal treatment for at least 3 months before surgery. Explanted endometrial tissue was cut into fragments of 1–2 mm in diameter under sterile conditions and left for 1 h in culture medium (DMEM-Ham's F12 1:1; Biochrom KG, Berlin, Germany) supplemented with Pen/Strep (Gibco BRL, Karlsruhe, Germany) at 37°C prior to transplantation into nude mice.Athymic female nude mice (Han:n MRI nu/nu) were maintained in a barrier unit in a controlled pathogen-free environment and regulated light/dark cycles (12/12 h).Each mouse received four endometrial tissue fragments which fixed intraperitoneally to the lateral abdominal wall of each nude mouse by laparotomy with surgical sutures as described before (Fechner ., 2007).Implanted endometrial lesions were dissected 7 (40 lesions/group) or 28 days (40 lesions/group) after transplantation, respectively. Two lesions of each mouse were frozen directly in liquid nitrogen for RNA extraction, and two lesions of each mouse were fixed with formalin and embedded in paraffin.Thus, progestins regulate factors important for the establishment and maintenance of ectopic endometrial lesions.Endometriosis, defined by the presence of endometrium-like tissues outside the uterus, is one of the most frequent benign gynaecological diseases and affects an estimated 10–15% of all women of reproductive age and 30% of women suffering from infertility or pelvic pain (Cramer and Missmer, 2002; Giudice and Kao, 2004).Progestogens are generally well-tolerated, have a limited metabolic impact compared with drugs interfering with the pituitary regulation, are inexpensive and may be used on a long-term basis.Besides progesterone, dydrogesterone, a potent, orally active progestogen indicated in a wide variety of gynaecological conditions, is used in the therapy of endometriosis (Overton ., 1994).Although dydrogesterone significantly reduced proliferation of endometrial stromal cells after 28 days, suppression of apoptosis was independent from progestins.Expression of MMP-2 was significantly reduced by all progestins and MMP-3 by dydrogesterone.